Validity of evaluating spinal kinetics without participant-specific kinematics.

Musculoskeletal models are commonly used to estimate in vivo spinal loads under various loading conditions. Typically, participant-specific measured kinematics (PSMK) are coupled with participant-specific models, but obtaining PSMK data can be costly and infeasible in large studies or clinical practice. Thus, we evaluated two alternative methods to estimate spinal loads without PSMK: 1) ensemble average kinematics (EAK) based on kinematics from all participants; and 2) using separately measured individual kinematics (SMIK) from multiple other participants as inputs, then averaging the resulting loads. This study compares the dynamic spine loading patterns and peak loads in older adults performing five lifting tasks using PSMK, EAK and SMIK. Median root mean square errors of EAK and SMIK methods versus PSMK ranged from 18 to 72% body weight for compressive loads and from 2 to 25% body weight for shear loads, with median cross-correlations ranging from 0.931 to 0.991. The root mean square errors and cross-correlations between repeated PSMK trials fell within similar ranges. Compressive peak loads evaluated by EAK and SMIK were not different than PSMK in 12 of 15 cases, while by comparison repeated PSMK trials were not different in 13 of 15 cases. Overall, the resulting spine loading magnitudes and profiles using EAK or SMIK were not notably different than using a PSMK approach, and differences were not greater than between two PSMK trials. Thus, these findings indicate that these approaches may be used to make reasonable estimates of dynamic spinal loading without direct measurement of participant kinematics.

Metabolic Cost of a Proposed NMES Spaceflight Countermeasure Compared to Walking in Active Adults.

INTRODUCTION: Astronauts exercise to reduce microgravity-induced bone loss, but the resultant skeletal loading may not be sufficient to reduce fracture risk on an extended Mars mission. Adding additional exercise increases the risk of a negative caloric balance. Neuromuscular electrical stimulation (NMES) induces involuntary muscle contractions, which load the skeleton. The metabolic cost of NMES is not fully understood. On Earth, walking is a common source of skeletal loading. If the metabolic cost of NMES were equal to or less than walking, it could offer a low metabolic cost option for increasing skeletal loading.METHODS:We measured the oxygen consumed and carbon dioxide produced from 10 subjects during 5-min bouts of walking at 2 mph, 3 mph, and 2 mph on a 6° incline, and of NMES to the legs at duty cycles of 1 s on and 5 s, 4 s, or 3 s off. Metabolic cost was calculated using the Brockway equation and the percent increase above resting for each NMES bout was compared to walking.RESULTS: Metabolic cost increased 64.9 ± 52.8% from rest in the most intense NMES duty cycle (1 s/3 s) and 120.4 ± 26.5%, 189.3 ± 59.5%, 281.7 ± 66.8%, for the 2 mph, 3 mph, and incline walking, respectively. The metabolic cost did not differ significantly between the three NMES duty cycles.DISCUSSION: The increase in metabolic cost of the fastest NMES bout was less than that of the slowest walk, indicating that numerous NMES bouts offer a way to increase skeletal loading at a modest metabolic cost. This might allow for more daily skeletal loading cycles, potentially further reducing bone loss.Abitante TJ, Alemi MM, Newman DJ, Duda KR. Metabolic cost of a proposed NMES spaceflight countermeasure compared to walking in active adults. Aerosp Med Hum Perform. 2023; 94(7):523-531.

EMG Validation of a Subject-Specific Thoracolumbar Spine Musculoskeletal Model During Dynamic Activities in Older Adults.

Musculoskeletal models can uniquely estimate in vivo demands and injury risk. In this study, we aimed to compare muscle activations from subject-specific thoracolumbar spine OpenSim models with recorded muscle activity from electromyography (EMG) during five dynamic tasks. Specifically, 11 older adults (mean = 65 years, SD = 9) lifted a crate weighted to 10% of their body mass in axial rotation, 2-handed sagittal lift, 1-handed sagittal lift, and lateral bending, and simulated a window opening task. EMG measurements of back and abdominal muscles were directly compared to equivalent model-predicted activity for temporal similarity via maximum absolute normalized cross-correlation (MANCC) coefficients and for magnitude differences via root-mean-square errors (RMSE), across all combinations of participants, dynamic tasks, and muscle groups. We found that across most of the tasks the model reasonably predicted temporal behavior of back extensor muscles (median MANCC = 0.92 ± 0.07) but moderate temporal similarity was observed for abdominal muscles (median MANCC = 0.60 ± 0.20). Activation magnitude was comparable to previous modeling studies, and median RMSE was 0.18 ± 0.08 for back extensor muscles. Overall, these results indicate that our thoracolumbar spine model can be used to estimate subject-specific in vivo muscular activations for these dynamic lifting tasks.

A Systematic Review on Lower-Limb Industrial Exoskeletons: Evaluation Methods, Evidence, and Future Directions.

Industrial tasks that involve frequent sitting/standing transitions and squatting activities can benefit from lower-limb industrial exoskeletons; however, their use is not as widespread as their upper-body counterparts. In this review, we examined 23 articles that evaluated the effects of using Wearable Chair (WC) and Squat-assist (SA) exoskeletons. Evaluations mainly included assessment of muscular demands in the thigh, shank, and upper/lower back regions. Both types of devices were found to lessen muscular demands in the lower body by 30-90%. WCs also reduced low-back demands (~ 37%) and plantar pressure (54-80%) but caused discomfort/unsafe feeling in participants. To generalize outcomes, we suggest standardizing approaches used for evaluating the devices. Along with addressing low adoption through design upgrades (e.g., ground and body supports/attachments), we recommend that researchers thoroughly evaluate temporal effects on muscle fatigue, metabolic rate, and stability of wearers. Although lower-limb exoskeletons were found to be beneficial, discrepancies in experimental protocols (posture/task/measures) were discovered. We also suggest simulating more realistic conditions, such as walking/sitting interchangeability for WCs and lifting loads for SA devices. The presented outcomes could help improve the design/evaluation approaches, and implementation of lower limb wearable devices across industries.

Recovering from Laboratory-Induced slips and trips causes high levels of lumbar muscle activity and spine loading.

Slips, trips, and falls are some of the most substantial and prevalent causes of occupational injuries and fatalities, and these events may contribute to low-back problems. We quantified lumbar kinematics (i.e., lumbar angles relative to pelvis) and kinetics during unexpected slip and trip perturbations, and during normal walking, among 12 participants (6F, 6 M). Individual anthropometry, lumbar muscle geometry, and lumbar angles, along with electromyography from 14 lumbar muscles were used as input to a 3D, dynamic, EMG-based model of the lumbar spine. Results indicated that, in comparison with values during normal walking, lumbar range of motion, lumbosacral (L5/S1) loads, and lumbar muscle activations were all significantly higher during the slip and trip events. Maximum L5/S1 compression forces exceeded 2700 N during slip and trip events, compared with âˆ¼ 1100 N during normal walking. Mean values of L5/S1 anteroposterior (930 N), and lateral (800 N) shear forces were also substantially larger than the shear force during the normal walking (230 N). These observed levels of L5/S1 reaction forces, along with high levels of bilateral lumbar muscle activities, suggest the potential for overexertion injuries and tissue damage during unexpected slip and trip events, which could contribute to low back injuries. Outcomes of this study may facilitate the identification and control of specific mechanisms involved with low back disorders consequent to slips or trips.

Using static postures to estimate spinal loading during dynamic lifts with participant-specific thoracolumbar musculoskeletal models.

Static biomechanical simulations are sometimes used to estimate in vivo kinetic demands because they can be solved efficiently, but this ignores any potential inertial effects. To date, comparisons between static and dynamic analyses of spinal demands have been limited to lumbar joint differences in young males performing sagittal lifts. Here we compare static and dynamic vertebral compressive and shear force estimates during axial, lateral, and sagittal lifting tasks across all thoracic and lumbar vertebrae in older men and women. Participant-specific thoracolumbar full-body musculoskeletal models estimated vertebral forces from recorded kinematics both with and without consideration of dynamic effects, at an identified frame of peak vertebral loading. Static analyses under-predicted dynamic compressive and resultant shear forces, by an average of about 16% for all three lifts across the thoracic and lumbar spine but were highly correlated with dynamic forces (average r2 > .95). The study outcomes have the potential to enable standard clinical and occupational estimates using static analyses.

A Systematic Review on Evaluation Strategies for Field Assessment of Upper-Body Industrial Exoskeletons: Current Practices and Future Trends.

With rising manual work demands, physical assistance at the workplace is crucial, wherein the use of industrial exoskeletons (i-EXOs) could be advantageous. However, outcomes of numerous laboratory studies may not be directly translated to field environments. To explore this discrepancy, we conducted a systematic review including 31 studies to identify and compare the approaches, techniques, and outcomes within field assessments of shoulder and back support i-EXOs. Findings revealed that the subjective approaches [i.e., discomfort (23), usability (22), acceptance/perspectives (21), risk of injury (8), posture (3), perceived workload (2)] were reported more common (27) compared to objective (15) approaches [muscular demand (14), kinematics (8), metabolic costs (5)]. High variability was also observed in the experimental methodologies, including control over activity, task physics/duration, sample size, and reported metrics/measures. In the current study, the detailed approaches, their subject-related factors, and observed trends have been discussed. In sum, a new guideline, including tools/technologies has been proposed that could be utilized for field evaluation of i-EXOs. Lastly, we discussed some of the common technical challenges experimenters face in evaluating i-EXOs in field environments. Efforts presented in this study seek to improve the generalizability in testing and implementing i-EXOs.

Modeling the metabolic reductions of a passive back-support exoskeleton.

Despite several attempts to quantify the metabolic savings resulting from the use of passive back-support exoskeletons (BSEs), no study has modeled the metabolic change while wearing an exoskeleton during lifting. The objectives of this study were to: 1) quantify the metabolic reductions due to the VT-Lowe's exoskeleton during lifting; and 2) provide a comprehensive model to estimate the metabolic reductions from using a passive BSE. In this study, 15 healthy adults (13 males, 2 females) of ages 20-34 yr (mean = 25.33, SD = 4.43) performed repeated freestyle lifting and lowering of an empty box and a box with 20% of their bodyweight. Oxygen consumption and metabolic expenditure data were collected. A model for metabolic expenditure was developed and fitted with the experimental data of two prior studies and the without-exoskeleton experimental results. The metabolic cost model was then modified to reflect the effect of the exoskeleton. The experimental results revealed that VT-Lowe's exoskeleton significantly lowered the oxygen consumption by ∼9% for an empty box and 8% for a 20% bodyweight box, which corresponds to a net metabolic cost reduction of ∼12% and ∼9%, respectively. The mean metabolic difference (i.e., without-exo minus with-exo) and the 95% confidence interval were 0.36 and (0.2-0.52) W/kg for 0% body weight and 0.43 and (0.18-0.69) W/kg for 20% body weight. Our modeling predictions for with-exoskeleton conditions were precise, with absolute freestyle prediction errors of <2.1%. The model developed in this study can be modified based on different study designs, and can assist researchers in enhancing designs of future lifting exoskeletons.NEW & NOTEWORTHY We present a new model of the metabolic cost of repetitive lifting, and how that is affected by wearing a passive back support exoskeleton. We compute the effective biomechanical efficiencies of moving the body and a carried load during lifting, and determine the effect of an exoskeleton's efficiency on its metabolic reduction. This model is useful for understanding the effects of exoskeletons on the body and for designing future exoskeletons.

The Influence of Kinematic Constraints on Model Performance During Inverse Kinematics Analysis of the Thoracolumbar Spine.

Motion analysis is increasingly applied to spine musculoskeletal models using kinematic constraints to estimate individual intervertebral joint movements, which cannot be directly measured from the skin surface markers. Traditionally, kinematic constraints have allowed a single spinal degree of freedom (DOF) in each direction, and there has been little examination of how different kinematic constraints affect evaluations of spine motion. Thus, the objective of this study was to evaluate the performance of different kinematic constraints for inverse kinematics analysis. We collected motion analysis marker data in seven healthy participants (4F, 3M, aged 27-67) during flexion-extension, lateral bending, and axial rotation tasks. Inverse kinematics analyses were performed on subject-specific models with 17 thoracolumbar joints allowing 51 rotational DOF (51DOF) and corresponding models including seven sets of kinematic constraints that limited spine motion from 3 to 9DOF. Outcomes included: (1) root mean square (RMS) error of spine markers (measured vs. model); (2) lag-one autocorrelation coefficients to assess smoothness of angular motions; (3) maximum range of motion (ROM) of intervertebral joints in three directions of motion (FE, LB, AR) to assess whether they are physiologically reasonable; and (4) segmental spine angles in static ROM trials. We found that RMS error of spine markers was higher with constraints than without (p < 0.0001) but did not notably improve kinematic constraints above 6DOF. Compared to segmental angles calculated directly from spine markers, models with kinematic constraints had moderate to good intraclass correlation coefficients (ICCs) for flexion-extension and lateral bending, though weak to moderate ICCs for axial rotation. Adding more DOF to kinematic constraints did not improve performance in matching segmental angles. Kinematic constraints with 4-6DOF produced similar levels of smoothness across all tasks and generally improved smoothness compared to 9DOF or unconstrained (51DOF) models. Our results also revealed that the maximum joint ROMs predicted using 4-6DOF constraints were largely within physiologically acceptable ranges throughout the spine and in all directions of motions. We conclude that a kinematic constraint with 5DOF can produce smooth spine motions with physiologically reasonable joint ROMs and relatively low marker error.

Kinematic effects of a passive lift assistive exoskeleton.

The VT-Lowe's exoskeleton was designed to help support the back during repetitive lifting tasks. This study focused on the kinematic differences between lifting with and without the exoskeleton (With-Exo and Without-Exo) over three different lifting styles (Freestyle, Squat, and Stoop) and two different box weights (0% and 20% of bodyweight). Twelve young and healthy males (Age 23.5 +/- 4.42 years; Height 179.33 +/- 6.37 cm; Weight 80.4 +/- 5.59 kg) participated in this study. Variables analyzed include the ankle and knee angles and angle between the Shoulder-Hip-Knee (SHK); the shoulder, elbow, and wrist heights; and the lifting speed and acceleration. The relationships between the torso angle, SHK angle, center of mass of the torso, torso torque, box height, as well as electromyography (EMG) data from a related study were also analyzed. On average, wearing the exoskeleton resulted in a 1.5 degree increase in ankle dorsiflexion, a 2.6 degree decrease in knee flexion, and a decrease of 2.3 degrees in SHK angle. Subjects' shoulder, elbow, and wrist heights were slightly higher while wearing the exoskeleton, and they lifted slightly more slowly while wearing the exoskeleton. Subjects moved more quickly while bending down as compared to standing up, and with the 0% bodyweight box as compared to the 20% bodyweight box. The values for Freestyle lifts generally fell in between Squat and Stoop lift styles or were not significantly different from Squat. EMG data from the leg muscles had relationships with torso torque while the back and stomach muscles showed no significant relationships.

Assessing the potential for "undesired" effects of passive back-support exoskeleton use during a simulated manual assembly task: Muscle activity, posture, balance, discomfort, and usability.

Back-support exoskeletons (BSEs) are wearable systems designed to reduce physical demands on the back, but which could have undesired effects beyond this design intention. Participants (n = 18) used two commercial BSEs to complete a brief (~15-20 s) simulated manual assembly task in varying conditions, with outcome measures that included: working posture, activity levels in "secondary" muscle groups (shoulders and thighs), perceived balance, discomfort, and usability. Using a BSE led to small and inconsistent changes in working postures (e.g., < ~14° change in lumbar flexion), muscular activity in the secondary muscle groups (<±2% of maximum voluntary isometric contractions), or perceived balance. Limitations in movement were reported for both BSEs, however, along with moderate levels of discomfort. Task-specific responses were evident for all outcome measures, though these depended on the specific BSE used and differed between genders in many cases. Future work should focus on interactions between a given user, BSE design, and task conditions.

Biomechanical assessment of two back-support exoskeletons in symmetric and asymmetric repetitive lifting with moderate postural demands.

Two passive back-support exoskeleton (BSE) designs were assessed in terms of muscular activity, energy expenditure, joint kinematics, and subjective responses. Eighteen participants (gender-balanced) completed repetitive lifting tasks in nine different conditions, involving symmetric and asymmetric postures and using two BSEs (along with no BSE as a control condition). Wearing both BSEs significantly reduced peak levels of trunk extensor muscle activity (by ~9-20%) and reduced energy expenditure (by ~8-14%). Such reductions, though, were more pronounced in the symmetric conditions and differed between the two BSEs tested. Participants reported lower perceived exertion using either BSE yet raised concerns regarding localized discomfort. Minimal changes in lifting behaviors were evident when using either BSE, and use of both BSEs led to generally positive usability ratings. While these results are promising regarding the occupational use of BSEs, future work is recommended to consider inter-individual differences to accommodate diverse user needs and preferences.

Quantification of Postures for Low-Height Object Manipulation Conducted by Manual Material Handlers in a Retail Environment.

Occupational Applications Manual material handlers performing stocking tasks spent substantial amounts of time in bent postures but used traditional stoops and squats infrequently. Instead, they often used split-legged stoops and squats, where one foot is further forward than the other, and one-legged ("golfer's") lifts. During object manipulation, the distance workers reached away from their body, and the height at which they manipulated objects, were correlated with the posture used by the worker. Workers also stayed in different postures for different lengths of time. It is likely that certain postures are more comfortable for the workers to remain in, provide additional mobility or operational radius, or require less energy to use. Understanding these factors in more detail could lead to improved worker training programs, where the postures taught not only have low injury risk but are comfortable so are actually adopted and used by the workers.

Technical Abstract Background Musculoskeletal disorders are relatively common among manual material handlers. This may be due in part to challenging postures used by workers. Purpose Studying the kinematics of manual material handlers in the workplace will provide quantitative data on how they move and what postures they adopt. With these data, some insights can be determined about why workers chose certain postures. Methods We conducted an on-site workplace study to capture the full-body kinematics of manual material handlers (stockers) using inertial measurement units. We organized the observed bends into six classes: stooping, fore-aft squatting, split-legged stooping with one-heel raised, split-legged stooping with no heels raised, one-legged lifting, and mixed lifting, which include multiple forms while remaining bent. These classes were based on a new general classification of bending and lifting postures that we developed, which enumerates all of the possible forms. We quantified how frequently and for what duration the workers bent and lifted, and determined how often they performed asymmetric motions while bending. We determined the range of motion of the hand positions during each bent posture, which provides a measure of the workspace afforded by the posture. Results Workers rarely used symmetric squats and infrequently used symmetric stoops typically studied in lab settings. Instead, they used a variety of different postures that have not been well-characterized. Of the 666 bending postures recorded during the experiment, 27.3% were stoops lifts, 22.1% were one-legged lifts, 20.3% were split-legged bends with both heels on the ground, and 12.3% were split-legged bends with a heel raised. Only 4.6% of the postures were squats and only one participant used this posture. Different bending postures were correlated with different ranges of hand position used in object manipulation. One-legged lifting corresponded to bends with the hands furthest away from the body along the sagittal axis. Conclusions While our study was exploratory, we observed many kinematic forms that have not been studied much in the past, such as split-legged stooping and one-legged lifting, suggesting that future work should be done to understand the biomechanics of these postures.

Effects of Two Passive Back-Support Exoskeletons on Muscle Activity, Energy Expenditure, and Subjective Assessments During Repetitive Lifting.

OBJECTIVE: The aim of this study was to explore the efficacy of two different passive back-support exoskeleton (BSE) designs during repetitive lifting in different postures. BACKGROUND: Although BSEs have been proposed as a potential intervention for reducing physical demands, limited information is available about the impacts of different exoskeleton designs in diverse work scenarios. METHOD: Eighteen participants (gender-balanced) performed lab-based simulations of repetitive lifting tasks. These tasks were performed in 12 different conditions, involving two BSEs and a control condition, two levels of lifting symmetry (symmetric and asymmetric), and two postures (standing and kneeling). Outcome measures described muscle activity and energy expenditure, along with perceived discomfort, balance, and usability. RESULTS: Using both BSEs significantly reduced peak activity of the trunk extensor muscles (by ~10%-28%) and energy expenditure (by ~4%-13%) in all conditions tested. Such reductions, though, were task dependent and differed between the two BSEs. In most of the tested conditions, using BSEs positively affected subjective responses regarding perceived exertion and usability. CONCLUSION: Our results suggest that the beneficial effects of a BSE are task specific and depend on the specific BSE design approach. More work is needed, though, to better characterize this task specificity and to determine the generalizability of BSE effects on objective and subjective outcomes for a wider range of conditions and users. APPLICATION: Our results provide new evidence to guide the selection and application of passive BSE designs in diverse lifting tasks.

Biomechanical Evaluation of Passive Back-Support Exoskeletons in a Precision Manual Assembly Task: "Expected" Effects on Trunk Muscle Activity, Perceived Exertion, and Task Performance.

OBJECTIVE: To assess the efficacy of two different passive back-support exoskeleton (BSE) designs, in terms of trunk muscle activity, perceived low-back exertion, and task performance. BACKGROUND: BSEs have the potential to be an effective intervention for reducing low-back physical demands, yet little is known about the impacts of different designs in work scenarios requiring varying degrees of symmetric and asymmetric trunk bending during manual assembly tasks. METHOD: Eighteen participants (gender balanced) completed lab-based simulations of a precision manual assembly task using a "grooved pegboard." This was done in 26 different conditions (20 unsupported; 6 supported, via a chair), which differed in vertical height, horizontal distance, and orientation. RESULTS: Using both BSEs reduced metrics of trunk muscle activity in many task conditions (≤47% reductions when using BackX™ and ≤24% reductions when using Laevo™). Such reductions, though, were more pronounced in the conditions closer to the mid-sagittal plane and differed between the two BSEs tested. Minimal effects on task completion times or ratings of perceived exertion were found for both BSEs. CONCLUSION: Our findings suggest that using passive BSEs can be beneficial for quasi-static manual assembly tasks, yet their beneficial effects can be task specific and specific to BSE design approaches. Further work is needed, though, to better characterize this task specificity and to assess the generalizability of different BSE design approaches in terms of physical demands, perceived exertion, and task performance. APPLICATION: These results can help guide the choice and application of passive BSE designs for diverse work scenarios involving nonneutral trunk postures.

A passive exoskeleton reduces peak and mean EMG during symmetric and asymmetric lifting.

The VT-Lowe's exoskeleton is a novel passive lift-assistive device designed to offload the back muscles during repetitive lifting. In this study, the effect of the exoskeleton on electromyographic (EMG) signals was investigated in four different lifting types (stoop, squat, freestyle and asymmetric) and two box weights (0% and 20% of body weight). Twelve young healthy adults ages 18-31 years (mean = 22.75, SD = 4.35) were participants. The EMG signals for twelve muscles (iliocostalis erector spinae (IL), longissimus erector spinae (LT), multifidus (MF), bicep femoris (BF), vastus lateralis (VL) and abdominal external oblique (AEO) muscles) were measured. The exoskeleton significantly decreased the peak and mean activity of back muscles (IL and LT) by 31.5% and 29.3%, respectively, for symmetric lifts and by 28.2% and 29.5%, respectively, for asymmetric lifts. The peak and mean EMG of leg muscles were significantly reduced by 19.1% and 14.1% during symmetric lifts, and 17.4% and 14.6% during asymmetric lifts. Although the exoskeleton reduced the activation of back and leg muscles, it slightly increased the activity of external oblique muscles, although this was not statistically significant. In conclusion, the exoskeleton is promising as a lift-assist device for manual material handlers and workers performing repetitive lifting.

Assessing the influence of a passive, upper extremity exoskeletal vest for tasks requiring arm elevation: Part II - "Unexpected" effects on shoulder motion, balance, and spine loading.

Adopting a new technology (exoskeletal vest designed to support overhead work) in the workplace can be challenging since the technology may pose unexpected safety and health consequences. A prototype exoskeletal vest was evaluated for potential unexpected consequences with a set of evaluation tests for: usability (especially, donning & doffing), shoulder range of motion (ROM), postural control, slip & trip risks, and spine loading during overhead work simulations. Donning/doffing the vest was easily done by a wearer alone. The vest reduced the max. shoulder abduction ROM by ∼10%, and increased the mean center of pressure velocity in the anteroposterior direction by ∼12%. However, vest use had minimal influences on trip-/slip-related fall risks during level walking, and significantly reduced spine loadings (up to ∼30%) especially during the drilling task. Use of an exoskeletal vest can be beneficial, yet the current evaluation tests should be expanded for more comprehensiveness, to enable the safe adoption of the technology.

Assessing the influence of a passive, upper extremity exoskeletal vest for tasks requiring arm elevation: Part I - "Expected" effects on discomfort, shoulder muscle activity, and work task performance.

Use of exoskeletal vests (designed to support overhead work) can be an effective intervention approach for tasks involving arm elevation, yet little is known on the potential beneficial impacts of their use on physical demands and task performance. This laboratory study (n = 12) evaluated the effects of a prototype exoskeletal vest during simulated repetitive overhead drilling and light assembly tasks. Anticipated or expected benefits were assessed, in terms of perceived discomfort, shoulder muscle activity, and task performance. Using the exoskeletal vest did not substantially influence perceived discomfort, but did decrease normalized shoulder muscle activity levels (e.g., ≤ 45% reduction in peak activity). Drilling task completion time decreased by nearly 20% with the vest, but the number of errors increased. Overall, exoskeletal vest use has the potential to be a new intervention for work requiring arm elevation; however, additional investigations are needed regarding potential unexpected or adverse influences (see Part II).

Three dimensional passivated-electrode insulator-based dielectrophoresis.

In this study, a 3D passivated-electrode, insulator-based dielectrophoresis microchip (3D πDEP) is presented. This technology combines the benefits of electrode-based DEP, insulator-based DEP, and three dimensional insulating features with the goal of improving trapping efficiency of biological species at low applied signals and fostering wide frequency range operation of the microfluidic device. The 3D πDEP chips were fabricated by making 3D structures in silicon using reactive ion etching. The reusable electrodes are deposited on second glass substrate and then aligned to the microfluidic channel to capacitively couple the electric signal through a 100 µm glass slide. The 3D insulating structures generate high electric field gradients, which ultimately increases the DEP force. To demonstrate the capabilities of 3D πDEP, Staphylococcus aureus was trapped from water samples under varied electrical environments. Trapping efficiencies of 100% were obtained at flow rates as high as 350 µl/h and 70% at flow rates as high as 750 µl/h. Additionally, for live bacteria samples, 100% trapping was demonstrated over a wide frequency range from 50 to 400 kHz with an amplitude applied signal of 200 Vpp. 20% trapping of bacteria was observed at applied voltages as low as 50 Vpp. We demonstrate selective trapping of live and dead bacteria at frequencies ranging from 30 to 60 kHz at 400 Vpp with over 90% of the live bacteria trapped while most of the dead bacteria escape.